Christel Logez

I have a PhD in life and health sciences.
I have worked for several years on the production and purification of recombinant membrane proteins, notably G protein-coupled receptors. I used molecular biology techniques for the construction of plasmids, and I evaluated different expression systems (acellular synthesis, E. coli bacteria, Pichia pastoris yeast, HEK, CHO and BHK mammalian cells transfected or infected). I purified the proteins by different chromatographic techniques and implemented biochemical and biophysical analysis techniques (SDS-PAGE, Western Blot, DLS, SEC-MALS, TSA, Optim Technology, SPR, MST).
Then I joined the vaccine division of Sanofi 10 years ago and worked on cell culture (CHO, HEK, Vero, Sf9 insect cells, duck cells), virus culture (rabies, yellow fever, influenza, lentivector, baculovirus) and bacterial culture (E. coli, pathogenic bacteria). I generated genetically modified organisms by transformation of E. coli bacteria, transient transfection of Sf9 cells, stable transfection of HEK cells, CRISPR on Vero cells, Transposon on CHO. I implemented analytical techniques like SDS-PAGE, Western Blot, infectious titration, determination of transfection rates, ELISA, flow cytometry, enzymatic assay, UHA titration).
Four years ago, I integrated the Sanofi mRNA CoE to internalize the production and purification of plasmids used as template for the synthesis of mRNA vaccines. I am responsible for the unit in charge of E. coli bank manufacturing and fermentation process development to produce pDNA. It includes writing documentation and compliance with instructions, and different investigations based on key process parameters, like media/feed compositions, fed batch approaches, etc.