Maximizing the Recovery of Plasmid DNA: Accelerating Workflow to Increase the Efficiency of Lysis & DNA Extraction
Time: 3:45 pm
day: Conference Day One
Details:
- Developing efficient lysis procedures tailored to plasmid DNA extraction to reduce genomic DNA contamination
- Controlling important parameters to reduce shearing stress on plasmid DNA and promote a high yield of unbroken plasmids
- Closely monitoring parameters such as pH, buffer choice, and salt concentrations to prepare the extract for purification
