Controlling Fermentation Environments to Reduce Variability for Reproducible, High-Quality Plasmid Supply

• Unpacking key challenges in upstream fermentation and parameters to monitor during fermentation
• Reducing fermentation stresses to maintain plasmid robustness, sequence integrity and minimize batch-to-batch variability
• Balancing growth and yield to maximize efficiency while preserving critical elements like polyA tails for mRNA and ITRs for viral vectors
• Evaluating media formulation, feeding strategies and culture conditions for optimal plasmid recovery

Speakers:

Christel Logez

Unit head CMC DNA Process Development / mRNA center

Sanofi