Explore the Agenda
7:00 am Check-In, Coffee & Light Breakfast
7:50 am Chair’s Opening Remarks
Weighing Plasmid & Enzymatic Approaches in the Evolving Landscape of DNA Production
8:00 am Closed-End DNA as a Cell Free Template for mRNA Production
- Enabling rapid, scalable cell-free DNA template production for mRNA manufacturing through closed-end DNA (ceDNA) technology
- Achieving mRNA of similar quality to traditional plasmid DNA template methods with ceDNA templates
- Generating starting templates, selecting process raw materials, and purifying ceDNA as key steps for ensuring high DNA and mRNA quality
8:30 am Accelerating Manufacturing with Alchemy™ Cell-Free Systems
- Alchemy™ cell‑free DNA technology provides a faster, cleaner, and efficient linear DNA template for mRNA production
- Aldevron delivers fully integrated, end‑to‑end mRNA manufacturing all under one quality system and within purpose‑built U.S. facilities.
- Decades of experience and broad platform capabilities (DNA, RNA, proteins) position Aldevron as a partner for therapeutic development
9:00 am From Template to Transcript: Impact of DNA Product Quality on mRNA Therapeutic Outcomes
- Designing DNA vectors to influence double-stranded RNA formation and improve downstream process performance
- Engineering a plasmid that impacts polyA tail length to maximize product quality
- Understanding the importance of pDNA as a starting material on mRNA product quality to inform plasmid design
9:30 am Speed Networking
Our dedicated speed networking session is the perfect opportunity to have in-depth conversations and forge long-lasting connections with fellow technical experts working with plasmid and synthetic DNA
10:00 am Morning Break & Refreshments
Unleashing Synthetic DNA Production with Novel Platforms & Streamlined Processes for Bacterial Contamination-Free Material
10:30 am Pure Synthetic DNA: Unlocking the Genetic Medicines Needs
- Quality comes first – Ensuring high quality products and reproducible results
- Faster Delivery Time – Accelerating access to DNA material through high performance manufacturing process
- Scalability – Meeting any amount demanded: from R&D and clinical to commercial
11:15 am Roundtable Discussion: Comparing DNA Production Methods: Chemical, Cell-Free Amplification, Enzymatic, Biological & Beyond
- Clarifying what “cell-free” production really entails
- Comparing structural and impurity profiles across different DNA production approaches
- Exploring scalability, process flexibility, and cost implications of each method
- Sharing practical experiences and lessons learned from implementing different DNA platforms
11:30 am Small but Mighty: Developing a Cost-Effective Manufacturing Process for High-Quality Plasmids at Small Scale
- Increasing demands for small-scale amounts of plasmid DNA for early-stage and personalized medicine projects
- Transitioning from a gram-scale to a mg-scale process without sacrificing critical quality attributes
- Streamlining process, QC testing and documentation to shorten manufacturing and release timelines
12:00 pm 4basebio Lunch & Learn
Private hosted lunch by 4basebio, including a roundtable discussion on leveraging cell-free synthetic DNA to de-risk CMC pathways
Refining Upstream Process Development, Plasmid Design & Fermentation to Maximize DNA Integrity, Stability & Yield
1:00 pm On-Demand Buffer Solutions for Next-Gen Therapies
- Build vs Buy decisions for reagent solutions
- Background on RUO/GMP buffer manufacturing capabilities to support R&D and CMC
- Case Study: Supporting US CDMO scale their operations with buffer outsourcing
1:10 pm Optimizing Synthetic DNA Template Design to Improve repRNA Yield & Quality
- Comparing synthetic vs plasmid-derived DNA templates
- Evaluating template quality and its correlation with RNA integrity
- Mitigating amplification-induced artifacts
- Establishing quality metrics for synthetic DNA-based IVT templates
1:40 pm Scaling Plasmid DNA Production
- Media and peptone selection: Understand how these cell culture components contribute to enhanced productivity and performance across various E. coli strains
- Process optimization: Gain insights into the best practices for achieving significant performance improvements in pDNA development
- Scalability in pDNA production: Discover how Gibco peptones enabled Akron Bio to scale pDNA production efficiently
2:10 pm Controlling Fermentation Environments to Reduce Variability for Reproducible, High-Quality Plasmid Supply
- Unpacking key challenges in upstream fermentation and parameters to monitor during fermentation
- Reducing fermentation stresses to maintain plasmid robustness, sequence integrity and minimize batch-to-batch variability
- Balancing growth and yield to maximize efficiency while preserving critical elements like polyA tails for mRNA and ITRs for viral vectors
- Evaluating media formulation, feeding strategies and culture conditions for optimal plasmid recovery
2:40 pm Afternoon Break & Poster Session
This is an informal session designed to help you connect with peers in a relaxed atmosphere while exploring the latest innovations in DNA production, purification, and analytics. With an audience of experts eager to discuss advancements in plasmid quality, enzymatic synthesis, and analytical testing, you will have the opportunity to display a poster presenting your own research. Don’t miss the chance to connect, learn, and showcase your work in DNA production.
Enhancing DNA Recovery in Downstream Processing to Enable Efficient, Scalable Therapeutic Production
3:30 pm Controlling Variability in Downstream Processing to Produce Quality Plasmid DNA at Industrial Scale
- Understanding how downstream processes impact DNA isoforms (supercoiled vs nicked/linear)
- Identifying the main causes of DNA damage during DSP: hold-up time and temperature during resuspension
- Exploring how DNA quality impacts stability
4:00 pm Column-Free DNA Production: Tackling Scalability Challenges
- Exploring poultry H5N1 vaccine breakthroughs using column-free processes with >95% supercoiled DNA and proven long-term stability
- Learning GMP scale-up lessons from 50L to 1000L fermentation batches while navigating regulatory requirements for novel platforms
- Analyzing process economics and applying real-world facility implementation strategies for resin versus resin-free production
4:30 pm Enhancing Plasmid Yield: Optimizing Lysis & Extraction for Maximum Recovery
- Tailoring lysis strategies to selectively release plasmid DNA while minimizing DNA carryover
- Fine-tuning critical process conditions to reduce shear stress and preserve plasmid integrity
- Scaling of the lysis
5:00 pm Chair’s Closing Remarks
5:15 pm Drinks Reception hosted by Syngoi & Artis BioSolutions
Kick off the evening with insights from Kate Broderick, followed by a relaxed networking reception. Connect with peers over drinks and continue the day’s discussions