Matevz Korenc

Company: Sartorius

Job title: Scientist

Seminars:

Optimization of Plasmid DNA (pDNA) Extraction via Lysis to Enhance Manufacturing Efficiency and QualitY 2:15 pm

Plasmid DNA (pDNA) isolation involves a crucial cell lysis step using a strong alkaline solution, followed by neutralization and precipitation to recover target pDNA while removing cellular contaminants like genomic DNA and proteins. Uncontrolled alkaline use and mechanical stress can degrade pDNA, and highly viscous solutions may cause heterogeneities or require extensive mixing, further compromising…Read more

day: Conference Day One
day: Day One